Electron microscopic study of Bacillus subtilis protoplast fusion

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Electron microscopic study of Bacillus subtilis protoplast fusion.

When protoplasts derived from sporulating cells of Bacillus subtilis were fused by exposure to polyethylene glycol (PEG) and fixed immediately thereafter, protoplasts with two enclosed prespores could be seen by electron microscope. The number of fusion events was greatly increased, and multiply fused protoplasts appeared, when the PEG-treated suspension was diluted in hypertonic broth and rein...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1979

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.137.3.1354-1361.1979